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25-Hydroxyvitamin D (25-OH-D)

25-Hydroxycalciferol; 25-OH-D; Cholecalciferol Metabolite; Vitamin D3 Metabolite
  • Test Code: 510300
  • TAT: 24 hours
  • CPT Code(s): 82306
  • *Turnaround time is defined as the time from which a specimen is received into the laboratory until the time results are released. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.

Specimen Requirements

Specimen Type

Serum


Volume:

1mL


Minimum Volume:

0.3 mL


Container:

Gold top or gel-barrier tube


Collection:

Separate serum or plasma from cells within one hour of collection.


Storage/Transport Conditions:

Refrigerate


Specimen Stability:

Room Temperature - 8 hours

Refrigerated - 4 days

Frozen - 24 weeks


Causes for Rejection:

Plasma specimen; gross hemolysis; gross lipemia; improper labeling; improper collection; insufficient volume


Test Details

Use:

Vitamin D essential for bone health, and can be used to diagnose issues with bone metabolism. Severe deficiency can lead to bone-malformation, muscle weakness and function. Deficiency can be caused by hyperparathyroidism.


Limitations:

Do not use specimens from patients receiving high biotin doses until more than 8 hours from last biotin administration. Rare case interference due to extremely high titers of antibodies to analyte-specific antibodies, streptavidin or ruthenium can occur. For diagnostic purposes, results should always be assessed in conjunction with patient's medical history, clinical examination, and other findings.


Methodology:

Electrochemiluminescence assay


Reference Range (Expected Values):

> 30 ng/mL


Reportable Range:

5-200 ng/mL


Sample Report

Scope Information

Scope:
25-Hydroxycalciferol; 25-OH-D; Cholecalciferol Metabolite; Vitamin D3 Metabolite
Applicable Diseases:
Vitamin D essential for bone health, and can be used to diagnose issues with bone metabolism. Severe deficiency can lead to bone-malformation, muscle weakness and function. Deficiency can be caused by hyperparathyroidism.
Drugs Involved:
82306