25-Hydroxyvitamin D (25-OH-D)
25-Hydroxycalciferol; 25-OH-D; Cholecalciferol Metabolite; Vitamin D3 Metabolite- Test Code: 510300
- TAT: 24 hours
- CPT Code(s): 82306
*Turnaround time is defined as the time from which a specimen is received into the laboratory until the time results are released. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Specimen Requirements
Specimen Type
Serum
Volume:
1mL
Minimum Volume:
0.3 mL
Container:
Gold top or gel-barrier tube
Collection:
Separate serum or plasma from cells within one hour of collection.
Storage/Transport Conditions:
Refrigerate
Specimen Stability:
Room Temperature - 8 hours
Refrigerated - 4 days
Frozen - 24 weeks
Causes for Rejection:
Plasma specimen; gross hemolysis; gross lipemia; improper labeling; improper collection; insufficient volume
Test Details
Use:
Vitamin D essential for bone health, and can be used to diagnose issues with bone metabolism. Severe deficiency can lead to bone-malformation, muscle weakness and function. Deficiency can be caused by hyperparathyroidism.
Limitations:
Do not use specimens from patients receiving high biotin doses until more than 8 hours from last biotin administration. Rare case interference due to extremely high titers of antibodies to analyte-specific antibodies, streptavidin or ruthenium can occur. For diagnostic purposes, results should always be assessed in conjunction with patient's medical history, clinical examination, and other findings.
Methodology:
Electrochemiluminescence assay
Reference Range (Expected Values):
> 30 ng/mL
Reportable Range:
5-200 ng/mL
Sample Report
Scope Information
- Scope:
- 25-Hydroxycalciferol; 25-OH-D; Cholecalciferol Metabolite; Vitamin D3 Metabolite
- Applicable Diseases:
- Vitamin D essential for bone health, and can be used to diagnose issues with bone metabolism. Severe deficiency can lead to bone-malformation, muscle weakness and function. Deficiency can be caused by hyperparathyroidism.
- Drugs Involved:
- 82306